Pathway analysis is a computational method used in bioinformatics and systems biology to study the biological pathways that are involved in various biological processes, such as diseases, drug responses, or cellular functions. It aims to identify and analyze the relationships between genes, proteins, and metabolites within a specific pathway and understand how they interact to regulate cellular processes. Pathway analysis typically involves the use of various statistical and computational tools to analyze high-throughput omics data, such as gene expression, protein-protein interactions, and metabolic pathways. By mapping experimental data onto known biological pathways, researchers can identify key pathways that are dysregulated in a particular condition and gain insights into the underlying mechanisms of disease or biological processes. Overall, pathway analysis is a powerful tool for interpreting complex biological data and uncovering the molecular mechanisms underlying biological processes, which can lead to the discovery of potential drug targets and biomarkers for various diseases.